29th June 2023
- Moved required MinKNOW settings from the "Priming and loading a flow cell" sections into "Data acquisition and basecalling".
- Ensured MinKNOW barcode settings are consistent with our recommendations in the "downstream analysis" section.
6/29/2023
MRT_9127_v110_revW_14Jul2021
English
MinION
29th June 2023
- Moved required MinKNOW settings from the "Priming and loading a flow cell" sections into "Data acquisition and basecalling".
- Ensured MinKNOW barcode settings are consistent with our recommendations in the "downstream analysis" section.
6/29/2023
MRT_9127_v110_revW_14Jul2021
English
PromethION
29th June 2023
- Moved required MinKNOW settings from the "Priming and loading a flow cell" sections into "Data acquisition and basecalling".
- Ensured MinKNOW barcode settings are consistent with our recommendations in the "downstream analysis" section.
6th December 2021
- Updated kit contents image with the updated vial label and name from SPRI to AMPure XP Beads (AXP)
- Removed snippet for MinKNOW settings in " Sequencing and data analysis: Data acquisition and basecalling" as the required settings for Midnight are default when the Midnight expansion is selected in 'Kit selection' when setting up a MinKNOW run
6th December 2021
- Updated kit contents image with the updated vial label and name from SPRI to AMPure XP Beads (AXP)
- Removed snippet for MinKNOW settings in " Sequencing and data analysis: Data acquisition and basecalling" as the required settings for Midnight are default when the Midnight expansion is selected in 'Kit selection' when setting up a MinKNOW run
6th December 2021
- Updated kit contents image with the updated vial label and name from SPRI to AMPure XP Beads (AXP)
- Removed snippet for MinKNOW settings in " Sequencing and data analysis: Data acquisition and basecalling" as the required settings for Midnight are default when the Midnight expansion is selected in 'Kit selection' when setting up a MinKNOW run
23rd November 2021
- Updated the 'Data acquisition and basecalling' section to include information for starting an automatic Midnight analysis run on GridION devices.
23rd November 2021
- Updated the 'Data acquisition and basecalling' section to include information for starting an automatic Midnight analysis run on GridION devices.
23rd November 2021
- Updated the 'Data acquisition and basecalling' section to include information for starting an automatic Midnight analysis run on GridION devices.
4th October 2021
- Included information outlining the differences between this protocol and another similar protocol in the 'Overview of the protocol' section
- Updated the PCR mastermix ratios as well as reducing the volume of RT product taken forward
4th October 2021
- Included information outlining the differences between this protocol and another similar protocol in the 'Overview of the protocol' section
- Updated the PCR mastermix ratios as well as reducing the volume of RT product taken forward
4th October 2021
- Included information outlining the differences between this protocol and another similar protocol in the 'Overview of the protocol' section
- Updated the PCR mastermix ratios as well as reducing the volume of RT product taken forward
9th Sept 2021
- Updated equipment and consumables to use a Qubit fluorometer reader rather than a plate reader.
- Qubit dsDNA BR Assay Kit has been changed to Qubit dsDNA HS Assay Kit in the equipment and consumables section and pooling and clean-up step
9th Sept 2021
- Updated equipment and consumables to use a Qubit fluorometer reader rather than a plate reader.
- Qubit dsDNA BR Assay Kit has been changed to Qubit dsDNA HS Assay Kit in the equipment and consumables section and pooling and clean-up step
9th Sept 2021
- Updated equipment and consumables to use a Qubit fluorometer reader rather than a plate reader.
- Qubit dsDNA BR Assay Kit has been changed to Qubit dsDNA HS Assay Kit in the equipment and consumables section and pooling and clean-up step
31st August 2021
- Updated step: Pooling samples and clean-up in the PromethION branch so that the DNA library is made up to 23 µl with Elution Buffer (EB), rather than 11 µl as per for MinION flow cells.
31st August 2021
- Updated step: Pooling samples and clean-up in the PromethION branch so that the DNA library is made up to 23 µl with Elution Buffer (EB), rather than 11 µl as per for MinION flow cells.
31st August 2021
- Updated step: Pooling samples and clean-up in the PromethION branch so that the DNA library is made up to 23 µl with Elution Buffer (EB), rather than 11 µl as per for MinION flow cells.