29th September 2025
- In 'Overview of the protocol', in the Table under 'Library preparation', the timing for priming and loading a flow cell was increased from 5 min to 10 min.
- In 'Equipment and consumables', the image of the LSK114 kit in single-use tube format was removed.
- In section 4, 'DNA repair and end-prep', a step to 'Check your flow cell' and in section 6, 'Priming and loading the flow cell', a step to 'Complete a flow cell check' were included with links for the flow cell check document.
- In section 4, 'End-prep', step 23, instructions were provided on how to resuspend the AMPure XP bead pellet with nuclease-free water.
- In section 6, 'Priming and loading the flow cell', the instruction for removing the flow cell out of the fridge was moved to the top of this section. And instructions for the single-use tube format of the LSK114 kit were removed from step 2.
- In section 7, 'Data acquisition and basecalling', screenshots for MinKNOW were removed as instructions are self-explanatory.
- In 'Troubleshooting', section 11, the video links for priming and loading a MinION Flow Cell were replaced with links for the PromethION Flow Cell.
8/20/2024
CFS_9207_v114_revC_15May2024
English
PromethION
31 July 2024
- Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase
