Ligation sequencing gDNA - Native Barcoding Kit 96 V14 (SQK-NBD114.96)

DateVersion numberLanguageDeviceChanges
Aug 21 2024NBE_9171_v114_revP_15Sep2022EnglishFlongle
21st August 2024 - Removed Early access loading now that kit is in "released" phase. - Removed reference to duplex sequencing. - Updated flow cell loading recommendations based off input fragment size.
Aug 21 2024NBE_9171_v114_revP_15Sep2022EnglishGridION
21st August 2024 - Removed Early access loading now that kit is in "released" phase. - Removed reference to duplex sequencing. - Updated flow cell loading recommendations based off input fragment size.
Aug 21 2024NBE_9171_v114_revP_15Sep2022EnglishMinION
21st August 2024 - Removed Early access loading now that kit is in "released" phase. - Removed reference to duplex sequencing. - Updated flow cell loading recommendations based off input fragment size.
Aug 21 2024NBE_9171_v114_revP_15Sep2022EnglishPromethION
21st August 2024 - Removed Early access loading now that kit is in "released" phase. - Removed reference to duplex sequencing. - Updated flow cell loading recommendations based off input fragment size.
Aug 21 2024NBE_9171_v114_revP_15Sep2022中文Flongle
21st August 2024 - Removed Early access loading now that kit is in "released" phase. - Removed reference to duplex sequencing. - Updated flow cell loading recommendations based off input fragment size.
Aug 21 2024NBE_9171_v114_revP_15Sep2022中文GridION
21st August 2024 - Removed Early access loading now that kit is in "released" phase. - Removed reference to duplex sequencing. - Updated flow cell loading recommendations based off input fragment size.
Aug 21 2024NBE_9171_v114_revP_15Sep2022中文MinION
21st August 2024 - Removed Early access loading now that kit is in "released" phase. - Removed reference to duplex sequencing. - Updated flow cell loading recommendations based off input fragment size.
Aug 21 2024NBE_9171_v114_revP_15Sep2022中文PromethION
21st August 2024 - Removed Early access loading now that kit is in "released" phase. - Removed reference to duplex sequencing. - Updated flow cell loading recommendations based off input fragment size.
August 21st, 2024NBE_9171_v114_revO_15Sep2022EnglishFlongle Download 4th October 2023 - Updated the format of the final step in the "Adapter ligation and clean-up" step for the checklist
August 21st, 2024NBE_9171_v114_revO_15Sep2022EnglishPromethion Download 4th October 2023 - Updated the format of the final step in the "Adapter ligation and clean-up" step for the checklist
August 21st, 2024NBE_9171_v114_revO_15Sep2022EnglishGridion Download 4th October 2023 - Updated the format of the final step in the "Adapter ligation and clean-up" step for the checklist
August 21st, 2024NBE_9171_v114_revO_15Sep2022EnglishMinion Download 4th October 2023 - Updated the format of the final step in the "Adapter ligation and clean-up" step for the checklist
October 4th, 2023NBE_9171_v114_revN_15Sep2022EnglishFlongle Download 2nd October 2023 - Updated "Adapter ligation and clean-up" to remove additional reference of NEBNext® Quick Ligation Reaction Buffer (NEB, B6058)
October 4th, 2023NBE_9171_v114_revN_15Sep2022EnglishPromethion Download 2nd October 2023 - Updated "Adapter ligation and clean-up" to remove additional reference of NEBNext® Quick Ligation Reaction Buffer (NEB, B6058)
October 4th, 2023NBE_9171_v114_revN_15Sep2022EnglishGridion Download 2nd October 2023 - Updated "Adapter ligation and clean-up" to remove additional reference of NEBNext® Quick Ligation Reaction Buffer (NEB, B6058)
October 4th, 2023NBE_9171_v114_revN_15Sep2022EnglishMinion Download 2nd October 2023 - Updated "Adapter ligation and clean-up" to remove additional reference of NEBNext® Quick Ligation Reaction Buffer (NEB, B6058)
October 2nd, 2023NBE_9171_v114_revM_15Sep2022EnglishGridion Download 26th September 2023 - Added duplex information to the Sequencing and data analysis section
October 2nd, 2023NBE_9171_v114_revM_15Sep2022EnglishMinion Download 26th September 2023 - Added duplex information to the Sequencing and data analysis section
September 26th, 2023NBE_9171_v114_revL_15Sep2022EnglishFlongle Download 21st September 2023 - Updated flow cell loading instructions to include light shield installation - Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step. - Format change in "Native barcode ligation" step to fix downloaded checklist.
September 26th, 2023NBE_9171_v114_revL_15Sep2022EnglishPromethion Download 21st September 2023 - Updated flow cell loading instructions to include light shield installation - Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step. - Format change in "Native barcode ligation" step to fix downloaded checklist.
September 26th, 2023NBE_9171_v114_revL_15Sep2022EnglishGridion Download 21st September 2023 - Updated flow cell loading instructions to include light shield installation - Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step. - Format change in "Native barcode ligation" step to fix downloaded checklist.
September 26th, 2023NBE_9171_v114_revL_15Sep2022EnglishMinion Download 21st September 2023 - Updated flow cell loading instructions to include light shield installation - Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step. - Format change in "Native barcode ligation" step to fix downloaded checklist.
September 19th, 2023NBE_9171_v114_revJ_15Sep2022EnglishFlongle Download 14th August 2023 - Updated the equipment and consumables with both formats of kits which are being shipped. - Updated the native barcoding ligation step to include instructions for both EDTA concentrations. - Updated flow cell flushing instructions to include instructions for the bottle format kit.
September 19th, 2023NBE_9171_v114_revJ_15Sep2022EnglishPromethion Download 14th August 2023 - Updated the equipment and consumables with both formats of kits which are being shipped. - Updated the native barcoding ligation step to include instructions for both EDTA concentrations. - Updated flow cell flushing instructions to include instructions for the bottle format kit.
September 19th, 2023NBE_9171_v114_revJ_15Sep2022EnglishGridion Download 14th August 2023 - Updated the equipment and consumables with both formats of kits which are being shipped. - Updated the native barcoding ligation step to include instructions for both EDTA concentrations. - Updated flow cell flushing instructions to include instructions for the bottle format kit.
September 19th, 2023NBE_9171_v114_revJ_15Sep2022EnglishMinion Download 14th August 2023 - Updated the equipment and consumables with both formats of kits which are being shipped. - Updated the native barcoding ligation step to include instructions for both EDTA concentrations. - Updated flow cell flushing instructions to include instructions for the bottle format kit.
September 14th, 2023NBE_9171_v114_revI_15Sep2022EnglishFlongle Download 30th June 2023 - Added Flongle branch to the protocol.
September 14th, 2023NBE_9171_v114_revI_15Sep2022EnglishPromethion Download 30th June 2023 - Added Flongle branch to the protocol.
September 14th, 2023NBE_9171_v114_revI_15Sep2022EnglishGridion Download 30th June 2023 - Added Flongle branch to the protocol.
September 14th, 2023NBE_9171_v114_revI_15Sep2022EnglishMinion Download 30th June 2023 - Added Flongle branch to the protocol.
April 5th, 2023NBE_9171_v114_revH_15Sep2022EnglishPromethion Download April 2023 - Updated the DNA repair end-prep step mastermix tip.
April 5th, 2023NBE_9171_v114_revH_15Sep2022EnglishGridion Download April 2023 - Updated the DNA repair end-prep step mastermix tip.
April 5th, 2023NBE_9171_v114_revH_15Sep2022EnglishMinion Download April 2023 - Updated the DNA repair end-prep step mastermix tip.
March 24th, 2023NBE_9171_v114_revG_15Sep2022EnglishPromethion Download March 2023 - Updated the DNA repair end-prep step to show the tip for making up an end-prep mastermix before combining the reagents. - Corrected the expected volumes for pooled samples and the reflected in the AXP volumes.
March 24th, 2023NBE_9171_v114_revG_15Sep2022EnglishGridion Download March 2023 - Updated the DNA repair end-prep step to show the tip for making up an end-prep mastermix before combining the reagents. - Corrected the expected volumes for pooled samples and the reflected in the AXP volumes.
March 24th, 2023NBE_9171_v114_revG_15Sep2022EnglishMinion Download March 2023 - Updated the DNA repair end-prep step to show the tip for making up an end-prep mastermix before combining the reagents. - Corrected the expected volumes for pooled samples and the reflected in the AXP volumes.
January 17th, 2023NBE_9171_v114_revF_15Sep2022EnglishPromethion Download January 2023 - Updated the "Priming and loading the PrometION flow cell" section to the updated version for correct kit reagent vial size.
January 17th, 2023NBE_9171_v114_revF_15Sep2022EnglishGridion Download January 2023 - Updated the "Priming and loading the PrometION flow cell" section to the updated version for correct kit reagent vial size.
January 17th, 2023NBE_9171_v114_revF_15Sep2022EnglishMinion Download January 2023 - Updated the "Priming and loading the PrometION flow cell" section to the updated version for correct kit reagent vial size.
January 6th, 2023NBE_9171_v114_revE_15Sep2022EnglishPromethion Download January 2023 - Changed the "Priming and loading the PrometION flow cell" section to the updated version.
January 6th, 2023NBE_9171_v114_revE_15Sep2022EnglishGridion Download January 2023 - Changed the "Priming and loading the PrometION flow cell" section to the updated version.
January 6th, 2023NBE_9171_v114_revE_15Sep2022EnglishMinion Download January 2023 - Changed the "Priming and loading the PrometION flow cell" section to the updated version.
October 20th, 2022NBE_9171_v114_revD_15Sep2022EnglishPromethion Download 20th October 2022 - EXP-NBA114 information updated with availability
October 20th, 2022NBE_9171_v114_revD_15Sep2022EnglishGridion Download 20th October 2022 - EXP-NBA114 information updated with availability
October 20th, 2022NBE_9171_v114_revD_15Sep2022EnglishMinion Download 20th October 2022 - EXP-NBA114 information updated with availability
October 18th, 2022NBE_9171_v114_revC_15Sep2022EnglishPromethion Download 18th October 2022 - Updated equipment and consumables to remove reference to the NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing
October 18th, 2022NBE_9171_v114_revC_15Sep2022EnglishGridion Download 18th October 2022 - Updated equipment and consumables to remove reference to the NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing
October 18th, 2022NBE_9171_v114_revC_15Sep2022EnglishMinion Download 18th October 2022 - Updated equipment and consumables to remove reference to the NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing
October 4th, 2022NBE_9171_v114_revB_15Sep2022EnglishPromethion Download 4th Oct 2022 - Updated kit 14 info sheet snippet and link
October 4th, 2022NBE_9171_v114_revB_15Sep2022EnglishGridion Download 4th Oct 2022 - Updated kit 14 info sheet snippet and link
October 4th, 2022NBE_9171_v114_revB_15Sep2022EnglishMinion Download 4th Oct 2022 - Updated kit 14 info sheet snippet and link
September 15th, 2022NBE_9171_v114_revA_15Sep2022EnglishPromethion Download
September 15th, 2022NBE_9171_v114_revA_15Sep2022EnglishGridion Download
September 15th, 2022NBE_9171_v114_revA_15Sep2022EnglishMinion Download