Ligation sequencing amplicons - Native Barcoding Kit 24 V14 (SQK-NBD114.24) (NBA_9168_v114_revR_30Jan2025)

DateVersionLanguageDeviceChanges
1/30/2025NBA_9168_v114_revR_30Jan2025EnglishGridION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025NBA_9168_v114_revR_30Jan2025EnglishMinION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025NBA_9168_v114_revR_30Jan2025EnglishPromethION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
12/12/2024NBA_9168_v114_revQ_12Dec2024EnglishGridION
 12 December 2024 - Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/12/2024NBA_9168_v114_revQ_12Dec2024EnglishMinION
 12 December 2024 - Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/12/2024NBA_9168_v114_revQ_12Dec2024EnglishPromethION
 12 December 2024 - Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
11/15/2024NBA_9168_v114_revP_20Nov2024EnglishGridION
November 2024 - Updated kit content information and image to include new kit format of SQK-NBD114.24 with plated native barcodes.
11/15/2024NBA_9168_v114_revP_20Nov2024EnglishMinION
November 2024 - Updated kit content information and image to include new kit format of SQK-NBD114.24 with plated native barcodes.
11/15/2024NBA_9168_v114_revP_20Nov2024EnglishPromethION
November 2024 - Updated kit content information and image to include new kit format of SQK-NBD114.24 with plated native barcodes.
8/21/2024NBA_9168_v114_revO_15Sep2022EnglishGridION
21st August 2024 - Removed Early access loading now that kit is in "released" phase. - Removed reference to duplex sequencing. - Updated flow cell loading recommendations based off input fragment size.
8/21/2024NBA_9168_v114_revO_15Sep2022EnglishMinION
21st August 2024 - Removed Early access loading now that kit is in "released" phase. - Removed reference to duplex sequencing. - Updated flow cell loading recommendations based off input fragment size.
8/21/2024NBA_9168_v114_revO_15Sep2022EnglishPromethION
21st August 2024 - Removed Early access loading now that kit is in "released" phase. - Removed reference to duplex sequencing. - Updated flow cell loading recommendations based off input fragment size.
August 21st, 2024NBA_9168_v114_revM_15Sep2022EnglishGridion Download 07th March 2024 - Updated library loading recommendations to 35-50 fmols.
August 21st, 2024NBA_9168_v114_revM_15Sep2022EnglishMinion Download 07th March 2024 - Updated library loading recommendations to 35-50 fmols.
August 21st, 2024NBA_9168_v114_revM_15Sep2022EnglishPromethion Download 07th March 2024 - Updated library loading recommendations to 35-50 fmols.
October 4th, 2023NBA_9168_v114_revL_15Sep2022EnglishPromethion Download 2nd October 2023 - Updated "Adapter ligation and clean-up" to remove additional reference of NEBNext® Quick Ligation Reaction Buffer (NEB, B6058)
October 4th, 2023NBA_9168_v114_revL_15Sep2022EnglishGridion Download 2nd October 2023 - Updated "Adapter ligation and clean-up" to remove additional reference of NEBNext® Quick Ligation Reaction Buffer (NEB, B6058)
October 4th, 2023NBA_9168_v114_revL_15Sep2022EnglishMinion Download 2nd October 2023 - Updated "Adapter ligation and clean-up" to remove additional reference of NEBNext® Quick Ligation Reaction Buffer (NEB, B6058)
October 2nd, 2023NBA_9168_v114_revK_15Sep2022EnglishPromethion Download 21st September 2023 - Updated flow cell loading instructions to include light shield installation - Format change in "Native barcode ligation" step to fix downloaded checklist.
October 2nd, 2023NBA_9168_v114_revK_15Sep2022EnglishGridion Download 21st September 2023 - Updated flow cell loading instructions to include light shield installation - Format change in "Native barcode ligation" step to fix downloaded checklist.
October 2nd, 2023NBA_9168_v114_revK_15Sep2022EnglishMinion Download 21st September 2023 - Updated flow cell loading instructions to include light shield installation - Format change in "Native barcode ligation" step to fix downloaded checklist.
September 19th, 2023NBA_9168_v114_revI_15Sep2022EnglishPromethion Download 14th August 2023 - Updated the equipment and consumables with both formats of kits which are being shipped. - Updated the native barcoding ligation step to include instructions for both EDTA concentrations. - Updated flow cell flushing instructions to include instructions for the bottle format kit.
September 19th, 2023NBA_9168_v114_revI_15Sep2022EnglishGridion Download 14th August 2023 - Updated the equipment and consumables with both formats of kits which are being shipped. - Updated the native barcoding ligation step to include instructions for both EDTA concentrations. - Updated flow cell flushing instructions to include instructions for the bottle format kit.
September 19th, 2023NBA_9168_v114_revI_15Sep2022EnglishMinion Download 14th August 2023 - Updated the equipment and consumables with both formats of kits which are being shipped. - Updated the native barcoding ligation step to include instructions for both EDTA concentrations. - Updated flow cell flushing instructions to include instructions for the bottle format kit.
September 14th, 2023NBA_9168_v114_revH_15Sep2022EnglishPromethion Download April 2023 - Added flow cell priming mix volumes to the "Priming and loading" steps
September 14th, 2023NBA_9168_v114_revH_15Sep2022EnglishGridion Download April 2023 - Added flow cell priming mix volumes to the "Priming and loading" steps
September 14th, 2023NBA_9168_v114_revH_15Sep2022EnglishMinion Download April 2023 - Added flow cell priming mix volumes to the "Priming and loading" steps
April 5th, 2023NBA_9168_v114_revG_15Sep2022EnglishPromethion Download April 2023 - Updated the DNA repair end-prep step mastermix tip.
April 5th, 2023NBA_9168_v114_revG_15Sep2022EnglishGridion Download April 2023 - Updated the DNA repair end-prep step mastermix tip.
April 5th, 2023NBA_9168_v114_revG_15Sep2022EnglishMinion Download April 2023 - Updated the DNA repair end-prep step mastermix tip.
March 24th, 2023NBA_9168_v114_revF_15Sep2022EnglishPromethion Download March 2023 - Updated end-prep step to show the tip for making up an end-prep mastermix before combining the reagents. - Corrected the expected volumes for pooled samples and the reflected in the AXP volumes.
March 24th, 2023NBA_9168_v114_revF_15Sep2022EnglishGridion Download March 2023 - Updated end-prep step to show the tip for making up an end-prep mastermix before combining the reagents. - Corrected the expected volumes for pooled samples and the reflected in the AXP volumes.
March 24th, 2023NBA_9168_v114_revF_15Sep2022EnglishMinion Download March 2023 - Updated end-prep step to show the tip for making up an end-prep mastermix before combining the reagents. - Corrected the expected volumes for pooled samples and the reflected in the AXP volumes.
January 6th, 2023NBA_9168_v114_revE_15Sep2022EnglishPromethion Download January 2023 - Changed the "Priming and loading the PrometION flow cell" section to the updated version.
January 6th, 2023NBA_9168_v114_revE_15Sep2022EnglishGridion Download January 2023 - Changed the "Priming and loading the PrometION flow cell" section to the updated version.
January 6th, 2023NBA_9168_v114_revE_15Sep2022EnglishMinion Download January 2023 - Changed the "Priming and loading the PrometION flow cell" section to the updated version.
October 20th, 2022NBA_9168_v114_revD_15Sep2022EnglishPromethion Download 20th October 2022 - EXP-NBA114 information updated with availability
October 20th, 2022NBA_9168_v114_revD_15Sep2022EnglishGridion Download 20th October 2022 - EXP-NBA114 information updated with availability
October 20th, 2022NBA_9168_v114_revD_15Sep2022EnglishMinion Download 20th October 2022 - EXP-NBA114 information updated with availability
October 18th, 2022NBA_9168_v114_revC_15Sep2022EnglishPromethion Download 18th October 2022 - Updated equipment and consumables to remove reference to the NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing
October 18th, 2022NBA_9168_v114_revC_15Sep2022EnglishGridion Download 18th October 2022 - Updated equipment and consumables to remove reference to the NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing
October 18th, 2022NBA_9168_v114_revC_15Sep2022EnglishMinion Download 18th October 2022 - Updated equipment and consumables to remove reference to the NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing
October 4th, 2022NBA_9168_v114_revB_15Sep2022EnglishPromethion Download 4th Oct 2022 - Updated kit 14 info sheet snippet and link
October 4th, 2022NBA_9168_v114_revB_15Sep2022EnglishGridion Download 4th Oct 2022 - Updated kit 14 info sheet snippet and link
October 4th, 2022NBA_9168_v114_revB_15Sep2022EnglishMinion Download 4th Oct 2022 - Updated kit 14 info sheet snippet and link
September 15th, 2022NBA_9168_v114_revA_15Sep2022EnglishPromethion Download -
September 15th, 2022NBA_9168_v114_revA_15Sep2022EnglishGridion Download -
September 15th, 2022NBA_9168_v114_revA_15Sep2022EnglishMinion Download -