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Ligation sequencing DNA V14 - automated Hamilton NGS STAR 96 (SQK-LSK114-XL) (GDA_9167_v114_revK_02Oct2025)


DateVersionLanguageDeviceChanges
10/2/2025GDA_9167_v114_revK_02Oct2025EnglishPromethION
2nd October 2025 - In 'Overview of the protocol', an important note on Kit14 sequencing and duplex basecalling info sheet was removed as this is a Legacy document and its information is no longer applicable. - In 'Priming and loading the PromethION Flow Cell', the step to remove the flow cell from the fridge was moved to the top of this section and a step to complete a flow cell check was included. - In 'Data acquisition and basecalling' information on duplex basecalling was removed as it is no longer applicable.
10/4/2023GDA_9167_v114_revJ_24Aug2022EnglishPromethION
4th October 2023 - Updated the format of the final step in the "Adapter ligation and clean-up" step for the checklist

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