A guide to transcriptomics with Oxford Nanopore

For insights into complex biological mechanisms, a deep understanding of RNA diversity within transcriptomes is key. Oxford Nanopore sequencing delivers high outputs of reads that span full-length transcripts, providing unambiguous isoform detection for isoform expression analysis, as well as robust gene expression profiles. We offer streamline, end-to-end workflows for bulk transcriptome analysis, through cDNA sequencing or direct RNA sequencing — the only available technique that directly reads single, native RNA molecules. This guide introduces cDNA and direct RNA nanopore sequencing, for ultra-rich transcriptomic data without compromise.

In this getting started guide, you will:

• Find out about the benefits of nanopore sequencing for bulk transcriptomics, and how it reveals isoform-level information missed by legacy short-read technologies

• Discover how to go from sample to answer with streamlined, end-to-end workflows for cDNA and direct RNA nanopore sequencing

• Find out about the range of PromethION sequencing devices

• Learn how nanopore transcriptomics data can be easily analysed using the EPI2ME data analysis workflow wf-transcriptomes

• Read real-world case studies describing how researchers are utilising cDNA and direct RNA nanopore sequencing to reveal hidden diversity in health and disease