Workflow: direct RNA sequencing

Detecting isoforms and RNA modifications with PCR-free, direct RNA nanopore sequencing

Accurately capturing the range of RNA diversity can help elucidate the molecular mechanisms of disease and functional roles of RNA modifications. Nanopore sequencing is the only available technology that directly reads native RNA transcripts, enabling quantitation of gene and isoform expression without PCR bias along with direct RNA methylation detection. This end-to-end workflow provides a simple method to characterise RNA modifications from a human blood research sample using direct RNA sequencing.

In this workflow, you will:

• Find out how direct RNA sequencing enhances isoform and RNA modification detection
• Discover our best practice sequencing workflow in detail, starting from the recommended extraction method, through to primary analysis
• Learn about our recommended sequencing kit and devices