Assessing variants across the human genome with interaction-free sequencing on PromethION

Overview

This protocol overview introduces the sequencing and analysis of variants across a whole human genome, using a PromethION to deliver high output without the need to interact with the sequencer once the sample is loaded. Ideal for those who are newer to nanopore sequencing or wish to minimise hands-on time, this end-to-end method generates sequencing data with a read length N50 of ~10 kb.

In this protocol overview, you will:

• Learn the key steps in the sequencing protocol
• Discover our sequencing workflow, starting from the recommended extraction method, through to primary analysis with EPI2ME
• Find out our recommended sequencing kit and devices