Infectious disease
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'This method will save time in obtaining high-quality complete genomes of bacteria for antimicrobial resistance surveillance, and is likely to become a valuable tool for monitoring the transmission of plasmid-borne drug resistance genes'
Zhao et al. Front. Microbiol. (2023)
Rapid access to results
Offering comprehensive, real-time insights into the genomics of infectious diseases — from pathogen identification and antimicrobial resistance (AMR) profiling to the assembly of high-quality genomes and variant identification — nanopore sequencing delivers immediate access to the critical genomic epidemiology data required to effectively control infectious disease outbreaks. Sequence in the lab or at sample source at a scale that suits your needs, with powerful portable and high-throughput nanopore sequencing devices.
Featured content
Pathogen metagenomics
Metagenomic sequencing is a key technique for the identification of potential pathogens. This end-to-end workflow overview introduces how to rapidly identify bacterial, fungal, and viral pathogens from respiratory research samples using metagenomic sequencing.
Delivering the future of genomic pathogen surveillance
Find out how nanopore sequencing overcomes the limitations of legacy genomic analysis technologies to deliver rapid, affordable, and informative results for a range of pathogen outbreaks, including SARS-CoV-2, Zika, Ebola, and many more — improving surveillance of infectious diseases.
AmPORE-TB
AmPORE-TB is a research workflow that delivers rapid characterisation of mutations associated with antimicrobial resistance in Mycobacterium tuberculosis, species identification, and lineage identification in a single assay, directly from sputum samples.
Combining fast library preparation with on-demand nanopore sequencing and hands-off, comprehensive analysis onboard the GridION AmPORE-TB device, the AmPORE-TB workflow can take as little as six hours for same-day results.
The GridION AmPORE-TB device, installed with the AmPORE-TB data analysis software, will be distributed by bioMérieux (Q-GRD-MK1-ATB).
Recommended device for infectious disease sequencing
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GridION
A compact benchtop device designed to run and analyse up to five MinION Flow Cells. Run multiple experiments on a single device, on-demand — ideal for rapid and scalable analysis of pathogen samples and tracking novel variants.
Featured analysis for infectious disease sequencing
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EPI2ME wf-flu
This workflow performs filtering, genetic variant calling, and sequence alignment to the CDC influenza reference. The analysis can be run using a fully automated, point-and-click user interface or via the command line.
Technology comparison
Oxford Nanopore sequencing
Legacy short-read sequencing
Any read length (50 bp to >4 Mb)
Short read length (<300 bp)
- Generate complete, high-quality genomes with fewer contigs and simplify de novo assembly
- Resolve genomic regions inaccessible to short reads, including complex structural variants (SVs) and repeats
- Analyse long-range haplotypes, accurately phase single nucleotide variants (SNVs) and base modifications, and identify parent-of-origin effects
- Sequence short DNA fragments, such as amplicons and cell-free DNA (cfDNA)
- Sequence and quantify full-length transcripts to annotate genomes, fully characterise isoforms, and analyse gene expression — including at single-cell resolution
- Resolve mobile genetic elements — including plasmids and transposons — to generate critical genomic insights
- Enhance taxonomic resolution using full-length reads of informative loci, such as the entire 16S gene
- Assembly contiguity is reduced and complex computational analyses are required to infer results
- Complex genomic regions such as SVs and repeat elements typically cannot be sequenced in single reads (e.g. transposons, gene duplications, and prophage sequences)
- Transcript analysis is limited to gene-level expression data
- Important genetic information is missed
Direct sequencing of native DNA/RNA
Amplification required
- Eliminate amplification- and GC-bias, along with read length limitations, and access genomic regions that are difficult to amplify
- Detect epigenetic modifications, such as methylation, as standard — no additional, time-consuming sample prep required
- Create cost-effective, amplification-free, targeted panels with adaptive sampling to detect SVs, repeats, SNVs, and methylation in a single assay
- Amplification is often required and can introduce bias
- Base modifications are removed, necessitating additional sample prep, sequencing runs, and expense
- Uniformity of coverage is reduced, resulting in assembly gaps
Real-time data streaming
Fixed run time with bulk data delivery
- Analyse data as it is generated for immediate access to actionable results
- Stop sequencing when sufficient data is obtained — wash and reuse flow cell
- Combine real-time data streaming with intuitive, real-time EPI2ME data analysis workflows for deeper insights
- Time to result is increased
- Workflow errors cannot be identified until it is too late
- Additional complexities of handling large volumes of bulk data
Accessible and affordable sequencing
Constrained to centralised labs
- Sequence on demand with flexible end-to-end workflows that suit your throughput needs
- Sequence at sample source, even in the most extreme or remote environments, with the portable MinION device — minimise potential sample degradation caused by storage and shipping
- Scale up with modular GridION and PromethION devices — suitable for high-output, high-throughput sequencing to generate ultra-rich data
- Sequence as and when needed using low-cost, independently addressable flow cells — no sample batching needed
- Use sample barcodes to multiplex samples on a single flow cell
- Bulky, expensive devices that require substantial site infrastructure — use is restricted to well-resourced, centralised locations, limiting global accessibility
- High sample batching is required for optimal efficiency, delaying time to results
Streamlined, automatable workflows
Laborious workflows
- Prepare samples in as little as 10 minutes, including multiplexing
- Use end-to-end whole-genome, metagenomic, targeted (including 16S barcoding), direct RNA and cDNA sequencing workflows
- Scale and automate your workflows to suit your sequencing needs
- Perform real-time enrichment of single targets or panels without additional wet-lab prep by using adaptive sampling
- Lengthy sample prep is required
- Long sequencing run times
- Workflow efficiency is reduced, and time to result is increased
