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Rapid sequencing DNA - PCR Barcoding Kit 24 V14 (SQK-RPB114.24) (RPB_9191_v114_revH_06Nov2025)

DateVersionLanguageDeviceChanges
11/6/2025RPB_9191_v114_revH_06Nov2025EnglishFlongle
6th November 2025 - In section 1, 'Compatibility of this protocol', MinION Mk1B and Mk1C were removed, along with their links for IT requirements as these device documents have moved to legacy. This also includes removal of links to their user manuals in 'Data acquisition and basecalling'. - In section 3, 'Library preparation' , a 'Check your flow cell' instruction was added to the start of the section. And a note was added to use the barcode wells only once. - In section 4, 'Priming and loading the MinION/GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. And the addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction. A note was added to the end of this section and for the Flongle Flow Cell loading to explain that when a flow cell is inserted into the MinION Mk1D, there will be a small gap around the sides of the device. - For the PromethION Flow Cell loading, the note to take the flow cell out of the fridge was moved to the top of this section. - The 'Data acquisition and basecalling' section was edited and formatted to align with current protocols.
11/6/2025RPB_9191_v114_revH_06Nov2025EnglishGridION
6th November 2025 - In section 1, 'Compatibility of this protocol', MinION Mk1B and Mk1C were removed, along with their links for IT requirements as these device documents have moved to legacy. This also includes removal of links to their user manuals in 'Data acquisition and basecalling'. - In section 3, 'Library preparation' , a 'Check your flow cell' instruction was added to the start of the section. And a note was added to use the barcode wells only once. - In section 4, 'Priming and loading the MinION/GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. And the addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction. A note was added to the end of this section and for the Flongle Flow Cell loading to explain that when a flow cell is inserted into the MinION Mk1D, there will be a small gap around the sides of the device. - For the PromethION Flow Cell loading, the note to take the flow cell out of the fridge was moved to the top of this section. - The 'Data acquisition and basecalling' section was edited and formatted to align with current protocols.
11/6/2025RPB_9191_v114_revH_06Nov2025EnglishMinION
6th November 2025 - In section 1, 'Compatibility of this protocol', MinION Mk1B and Mk1C were removed, along with their links for IT requirements as these device documents have moved to legacy. This also includes removal of links to their user manuals in 'Data acquisition and basecalling'. - In section 3, 'Library preparation' , a 'Check your flow cell' instruction was added to the start of the section. And a note was added to use the barcode wells only once. - In section 4, 'Priming and loading the MinION/GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. And the addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction. A note was added to the end of this section and for the Flongle Flow Cell loading to explain that when a flow cell is inserted into the MinION Mk1D, there will be a small gap around the sides of the device. - For the PromethION Flow Cell loading, the note to take the flow cell out of the fridge was moved to the top of this section. - The 'Data acquisition and basecalling' section was edited and formatted to align with current protocols.
11/6/2025RPB_9191_v114_revH_06Nov2025EnglishPromethION
6th November 2025 - In section 1, 'Compatibility of this protocol', MinION Mk1B and Mk1C were removed, along with their links for IT requirements as these device documents have moved to legacy. This also includes removal of links to their user manuals in 'Data acquisition and basecalling'. - In section 3, 'Library preparation' , a 'Check your flow cell' instruction was added to the start of the section. And a note was added to use the barcode wells only once. - In section 4, 'Priming and loading the MinION/GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. And the addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction. A note was added to the end of this section and for the Flongle Flow Cell loading to explain that when a flow cell is inserted into the MinION Mk1D, there will be a small gap around the sides of the device. - For the PromethION Flow Cell loading, the note to take the flow cell out of the fridge was moved to the top of this section. - The 'Data acquisition and basecalling' section was edited and formatted to align with current protocols.
5/29/2025RPB_9191_v114_revG_07May2025EnglishFlongle
08th May 2025 - Added information for SQK-RPB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Rapid Barcode Primers at 10 μM, new plate format with Rapid Barcode Primers at 1 μM.
5/29/2025RPB_9191_v114_revG_07May2025EnglishGridION
08th May 2025 - Added information for SQK-RPB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Rapid Barcode Primers at 10 μM, new plate format with Rapid Barcode Primers at 1 μM.
5/29/2025RPB_9191_v114_revG_07May2025EnglishMinION
08th May 2025 - Added information for SQK-RPB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Rapid Barcode Primers at 10 μM, new plate format with Rapid Barcode Primers at 1 μM.
5/29/2025RPB_9191_v114_revG_07May2025EnglishPromethION
08th May 2025 - Added information for SQK-RPB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Rapid Barcode Primers at 10 μM, new plate format with Rapid Barcode Primers at 1 μM.
5/7/2025RPB_9191_v114_revG_07May2025EnglishFlongle
08th May 2025 - Added information for SQK-RPB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Rapid Barcode Primers at 10 μM, new plate format with Rapid Barcode Primers at 1 μM.
5/7/2025RPB_9191_v114_revG_07May2025EnglishGridION
08th May 2025 - Added information for SQK-RPB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Rapid Barcode Primers at 10 μM, new plate format with Rapid Barcode Primers at 1 μM.
5/7/2025RPB_9191_v114_revG_07May2025EnglishMinION
08th May 2025 - Added information for SQK-RPB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Rapid Barcode Primers at 10 μM, new plate format with Rapid Barcode Primers at 1 μM.
5/7/2025RPB_9191_v114_revG_07May2025EnglishPromethION
08th May 2025 - Added information for SQK-RPB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Rapid Barcode Primers at 10 μM, new plate format with Rapid Barcode Primers at 1 μM.
1/28/2025RPB_9191_v114_revF_11Dec2024EnglishFlongle
28th January 2025 - In the Flongle branch of "Equipment and consumables", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
1/28/2025RPB_9191_v114_revF_11Dec2024EnglishGridION
28th January 2025 - In the Flongle branch of "Equipment and consumables", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
1/28/2025RPB_9191_v114_revF_11Dec2024EnglishMinION
28th January 2025 - In the Flongle branch of "Equipment and consumables", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
1/28/2025RPB_9191_v114_revF_11Dec2024EnglishPromethION
28th January 2025 - In the Flongle branch of "Equipment and consumables", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
12/11/2024RPB_9191_v114_revE_11Dec2024EnglishFlongle
11th December 2024 - Addition of link to MinION Mk1D IT requirements in "Compatibility of this protocol" and links to MinION Mk1B/Mk1D user manuals in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link to EPI2ME in "Downstream analysis", "EPI2ME workflows". - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/11/2024RPB_9191_v114_revE_11Dec2024EnglishGridION
11th December 2024 - Addition of link to MinION Mk1D IT requirements in "Compatibility of this protocol" and links to MinION Mk1B/Mk1D user manuals in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link to EPI2ME in "Downstream analysis", "EPI2ME workflows". - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/11/2024RPB_9191_v114_revE_11Dec2024EnglishMinION
11th December 2024 - Addition of link to MinION Mk1D IT requirements in "Compatibility of this protocol" and links to MinION Mk1B/Mk1D user manuals in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link to EPI2ME in "Downstream analysis", "EPI2ME workflows". - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/11/2024RPB_9191_v114_revE_11Dec2024EnglishPromethION
11th December 2024 - Addition of link to MinION Mk1D IT requirements in "Compatibility of this protocol" and links to MinION Mk1B/Mk1D user manuals in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link to EPI2ME in "Downstream analysis", "EPI2ME workflows". - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
10/16/2024RPB_9191_v114_revD_16Oct2024EnglishFlongle
16th October 2024 - Updated Introduction to the protocol to the slimmed down structure. - Updated reagent use from LongAmp Taq 2X Master Mix (e.g. NEB, cat # M0287) to newly recommended LongAmp Hot Start Taq 2X Master Mix (NEB, M0533).
10/16/2024RPB_9191_v114_revD_16Oct2024EnglishGridION
16th October 2024 - Updated Introduction to the protocol to the slimmed down structure. - Updated reagent use from LongAmp Taq 2X Master Mix (e.g. NEB, cat # M0287) to newly recommended LongAmp Hot Start Taq 2X Master Mix (NEB, M0533).
10/16/2024RPB_9191_v114_revD_16Oct2024EnglishMinION
16th October 2024 - Updated Introduction to the protocol to the slimmed down structure. - Updated reagent use from LongAmp Taq 2X Master Mix (e.g. NEB, cat # M0287) to newly recommended LongAmp Hot Start Taq 2X Master Mix (NEB, M0533).
10/16/2024RPB_9191_v114_revD_16Oct2024EnglishPromethION
16th October 2024 - Updated Introduction to the protocol to the slimmed down structure. - Updated reagent use from LongAmp Taq 2X Master Mix (e.g. NEB, cat # M0287) to newly recommended LongAmp Hot Start Taq 2X Master Mix (NEB, M0533).
9/17/2024RPB_9191_v114_revC_17Sep2024EnglishFlongle
17th September 2024 - Protocol checklist now available. - Included clarification in the PCR amplification conditions that 14 cycles is recommended as a starting point but can be adjusted depending on experiment needs.
9/17/2024RPB_9191_v114_revC_17Sep2024EnglishGridION
17th September 2024 - Protocol checklist now available. - Included clarification in the PCR amplification conditions that 14 cycles is recommended as a starting point but can be adjusted depending on experiment needs.
9/17/2024RPB_9191_v114_revC_17Sep2024EnglishMinION
17th September 2024 - Protocol checklist now available. - Included clarification in the PCR amplification conditions that 14 cycles is recommended as a starting point but can be adjusted depending on experiment needs.
9/17/2024RPB_9191_v114_revC_17Sep2024EnglishPromethION
17th September 2024 - Protocol checklist now available. - Included clarification in the PCR amplification conditions that 14 cycles is recommended as a starting point but can be adjusted depending on experiment needs.
9/19/2023RPB_9191_v114_revB_28Jun2023EnglishFlongle
19th September 2023 - Updated flow cell loading instructions to include light shield installation
9/19/2023RPB_9191_v114_revB_28Jun2023EnglishGridION
19th September 2023 - Updated flow cell loading instructions to include light shield installation
9/19/2023RPB_9191_v114_revB_28Jun2023EnglishMinION
19th September 2023 - Updated flow cell loading instructions to include light shield installation
9/19/2023RPB_9191_v114_revB_28Jun2023EnglishPromethION
19th September 2023 - Updated flow cell loading instructions to include light shield installation
September 19th, 2023RPB_9191_v114_revA_28Jun2023EnglishFlongle Download -
September 19th, 2023RPB_9191_v114_revA_28Jun2023EnglishPromethion Download -
September 19th, 2023RPB_9191_v114_revA_28Jun2023EnglishGridion Download -
September 19th, 2023RPB_9191_v114_revA_28Jun2023EnglishMinion Download -

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