11 Dec 2024
- Updated kit content to include information on different RCS vial concentration in new kit batches.
- Updated instructions to include options for lower RCS concentration (old) and higher RCS concentraction (new).
- Addition of links to MinION Mk1D IT requirements and user manual.
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for EPI2ME in "Downstream analysis".
12/11/2024
DRCE_9196_v4_revC_11Dec2024
English
MinION
11 Dec 2024
- Updated kit content to include information on different RCS vial concentration in new kit batches.
- Updated instructions to include options for lower RCS concentration (old) and higher RCS concentraction (new).
- Addition of links to MinION Mk1D IT requirements and user manual.
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for EPI2ME in "Downstream analysis".
12/11/2024
DRCE_9196_v4_revC_11Dec2024
English
PromethION
11 Dec 2024
- Updated kit content to include information on different RCS vial concentration in new kit batches.
- Updated instructions to include options for lower RCS concentration (old) and higher RCS concentraction (new).
- Addition of links to MinION Mk1D IT requirements and user manual.
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for EPI2ME in "Downstream analysis".
7/31/2024
DRCE_9196_v4_revB_20Sep2023
English
GridION
31 July 2024
- Updated the protocol format to the new "slimmed-down" introduction sections.
- Updated workflow image in introduction to fix formatting errors in PDF generation
- Updated the Reverse transcriptase reagents and steps to the new method using Induro® Reverse Transcriptase (NEB, cat # M0681).
- Updated the RNase Inhibitor reagents and steps to the new method using Murine RNase Inhibitor (NEB, M0314).
- Updated first Agencourt RNAClean XP bead clean in Library Preparation section to elute into 23 ul Nuclease-free water, retain the full 23 ul and no longer have to add 3 ul of water in the next step.
- Updated "Flow cell reuse and returns" section to specify compatibility and use-cases for the Flow Cell Wash Kit with the RNA sequencing kit and RNA flow cells.
7/31/2024
DRCE_9196_v4_revB_20Sep2023
English
MinION
31 July 2024
- Updated the protocol format to the new "slimmed-down" introduction sections.
- Updated workflow image in introduction to fix formatting errors in PDF generation
- Updated the Reverse transcriptase reagents and steps to the new method using Induro® Reverse Transcriptase (NEB, cat # M0681).
- Updated the RNase Inhibitor reagents and steps to the new method using Murine RNase Inhibitor (NEB, M0314).
- Updated first Agencourt RNAClean XP bead clean in Library Preparation section to elute into 23 ul Nuclease-free water, retain the full 23 ul and no longer have to add 3 ul of water in the next step.
- Updated "Flow cell reuse and returns" section to specify compatibility and use-cases for the Flow Cell Wash Kit with the RNA sequencing kit and RNA flow cells.
7/31/2024
DRCE_9196_v4_revB_20Sep2023
English
PromethION
31 July 2024
- Updated the protocol format to the new "slimmed-down" introduction sections.
- Updated workflow image in introduction to fix formatting errors in PDF generation
- Updated the Reverse transcriptase reagents and steps to the new method using Induro® Reverse Transcriptase (NEB, cat # M0681).
- Updated the RNase Inhibitor reagents and steps to the new method using Murine RNase Inhibitor (NEB, M0314).
- Updated first Agencourt RNAClean XP bead clean in Library Preparation section to elute into 23 ul Nuclease-free water, retain the full 23 ul and no longer have to add 3 ul of water in the next step.
- Updated "Flow cell reuse and returns" section to specify compatibility and use-cases for the Flow Cell Wash Kit with the RNA sequencing kit and RNA flow cells.