DNA library preparation
Gain a comprehensive insight into DNA
A wide range of library preparation kits is available to suit whole-genome as well as targeted sequencing requirements. Amplification-free kits allow direct sequencing of short to ultra-long fragments of native DNA, eliminating PCR bias and allowing the detection of base modifications alongside standard nucleotides. Amplification-based kits enable whole-genome sequencing from low-input amounts or low-quality DNA (e.g. FFPE).
| Output optimised | Speed optimised | Ultra-long-read optimised | Low-input optimised | Targeted sequencing | |
|---|---|---|---|---|---|
| Ligation Sequencing Kit | Rapid Sequencing Kit | Ultra-long DNA Sequencing Kit | Rapid PCR Barcoding Kit | Microbial Amplicon Barcoding Kit | |
| Preparation time | 65 minutes | 10 minutes | 200 mins + 1x O/N incubation | 60 mins + PCR | 60 mins + PCR |
| Input recommendation | ~1,000 ng gDNA or 100–200 fmol for amplicons | ~100–200 ng gDNA | 6 M cells | 1–5 ng gDNA | 10 ng gDNA |
| Fragmentation | Optional | Transposase-based | Transposase-based | Transposase-based | - |
| Amplification | No | No | No | Yes | Yes |
| Multiplexing options | Native Barcoding Kit 24 Native Barcoding Kit 96 | Rapid Barcoding Kit 24 Rapid Barcoding Kit 96 | - | 24 plex | 24 plex |
| Read length | Equal to fragment length | Random distribution, dependent on input fragment length | N50 >50 kb | ~4–5 kb | Full-length 16S gene and ITS region |
| Adaptive Sampling | Yes | Yes | Yes | Yes | - |
| Methylation included | Yes | Yes | Yes | - | - |
Learn more about Adaptive Sampling
- A unique on-device, real-time targeted sequencing approach
- No probes or baits required
- Targeted sequencing plus low-pass whole-genome data, methylation, and phasing, in one run
Unlock high-accuracy direct sequencing
- Highly accurate detection of genetic variation
- Gold-standard methylation detection
- Reference-quality genome assemblies on a single platform
