Metagenomics resources

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Nanopore-only Microbial Isolate Sequencing Solution (NO-MISS) – automated ElysION (SQK-RBK114.96)

This end to end protocol describes our automated Nanopore-Only Microbial Isolate Sequencing Solution (NO-MISS): a flexible approach allowing sequencing of up to 24 microbial isolate genomes per MinION ...

October 7, 2024

Direct-from-colony rapid microbial sequencing for Salmonella serotyping using SQK-RPB114.24

A rapid method to perform sequencing and analysis of Salmonella.

August 28, 2024

Amplicon sequencing from DNA using SQK-RBK114 (.24 or .96)

This is the fastest and simplest protocol to sequence amplicon DNA. For Research Use Only Document version: RAA_9198_v114_revM_17Oct2025

November 14, 2023

Rapid metagenomic sequencing for surveillance of bacterial, fungal and viral pathogens using SQK-RPB114.24

This is a rapid method to perform metagenomic sequencing for identification of bacterial, fungal and viral pathogens. For Research Use Only

June 22, 2023

Plasmid sequencing from DNA using SQK-RBK114 (.24 or .96)

This protocol describes how to carry out rapid barcoding of plasmid DNA using the Rapid Barcoding Kit 24 or 96 V14 (SQK-RBK114.24 or SQK-RBK114.96) to sequence ...

April 25, 2023

Rapid sequencing DNA - Viral metagenomics for respiratory samples and skin lesion swabs (SQK-RPB004)

This is a legacy protocol describing rapid sequencing of respiratory samples and skin lesion swabs using the SQK-RPB004 kit.

May 31, 2022

Rapid sequencing plasmids (SQK-RBK004)

This is a legacy protocol describing sequencing of plasmids using the SQK-RBK004 kit.

July 29, 2021

PCR tiling of SARS-CoV-2 virus - automated Hamilton NGS STAR 96 (SQK-LSK109 with EXP-NBD196)

This is a legacy protocol describing sequencing of SARS-CoV-2 using the SQK-LSK109 kit.

November 13, 2020

PCR tiling of SARS-CoV-2 virus - classic protocol (SQK-LSK109 with EXP-NBD104, EXP-NBD114 or EXP-NBD196)

This is a legacy protocol describing sequencing of SARS-CoV-2 using the SQK-LSK109 kit with EXP-NBD104, EXP-NBD114 or EXP-NBD196.

February 5, 2020

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